A CUC triplet confers leucine-dependent regulation of the Bacillus subtilis ilv-leu operon

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A CUC triplet confers leucine-dependent regulation of the Bacillus subtilis ilv-leu operon.

Regulation of the ilv-leu operon probably involves interaction of a tR NA(GAG) with leader region mRNA. Conversion of a CUC (Leu) triplet located within the leader region to UUC (Phe), CGC (Arg), or UAC (Tyr) converted reporter gene expression to control by corresponding amino acids. Conversion of the CUC triplet to CUU (Leu) decreased expression and disrupted regulation. The results suggested ...

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Regions of the Bacillus subtilis ilv-leu operon involved in regulation by leucine.

The ilv-leu operon of Bacillus subtilis is regulated in part by transcription attenuation. The cis-acting elements required for regulation by leucine lie within a 683-bp fragment of DNA from the region upstream of ilvB, the first gene of the operon. This fragment contains the ilv-leu promoter and 482 bp of the ilv-leu leader region. Spontaneous mutations that lead to increased expression of the...

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Negative transcriptional regulation of the ilv-leu operon for biosynthesis of branched-chain amino acids through the Bacillus subtilis global regulator TnrA.

The Bacillus subtilis ilv-leu operon is involved in the synthesis of branched-chain amino acids (valine, isoleucine, and leucine). The two- to threefold repression of expression of the ilv-leu operon during logarithmic-phase growth under nitrogen-limited conditions, which was originally detected by a DNA microarray analysis to compare the transcriptomes from the wild-type and tnrA mutant strain...

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CcpA - Mediated Catabolite Activation of the Bacillus subtilis ilv - leu Operon 1 and Its Negation by Either CodY - or TnrA - Mediated Negative Regulation

CcpA-Mediated Catabolite Activation of the Bacillus subtilis ilv-leu Operon 1 and Its Negation by Either CodYor TnrA-Mediated Negative Regulation 2 3 4 Yasutaro Fujita*, Takenori Satomura, Shigeo Tojo, and Kazutake Hirooka 5 6 7 Department of Biotechnology, Faculty of Life Science and Biotechnology, Fukuyama 8 University, 985 Sanzo, Higashimura-cho, Fukuyama-shi, Hiroshima 729-0292, Japan 9 10 ...

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CcpA-mediated catabolite activation of the Bacillus subtilis ilv-leu operon and its negation by either CodY- or TnrA-mediated negative regulation.

The Bacillus subtilis ilv-leu operon functions in the biosynthesis of branched-chain amino acids. It undergoes catabolite activation involving a promoter-proximal cre which is mediated by the complex of CcpA and P-Ser-HPr. This activation of ilv-leu expression is negatively regulated through CodY binding to a high-affinity site in the promoter region under amino acid-rich growth conditions, and...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1996

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.178.7.2150-2153.1996